Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: VGEM 2013
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:08:42
ftsZ gene from E. coli on pSB1C3
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1011000_sequence (Version 1) |
Description
This is an E. coli ftsZ gene. FtsZ is a protein that polymerizes into the Z ring that is formed at the septum site during cellular division. The over expression of ftsZ will lead to non-specific septum sites at the polar ends, which result in the production of E. coli mini-cells.Notes
There is an illegal EcoR1 restriction site at bp 102-107 of the gene. We changed the C at site 107 to a T, which maintained the codon optimization and removed the illegal restriction site.Source
The organism for which we based our sequence is Escherichia coli str. K-12 substr. MG1655, located at the following NCBI url:http://www.ncbi.nlm.nih.gov/nuccore/NC_000913.2?report=genbank&from=105305&to=106456