Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Maithili Krishnan
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:08:45
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1022120_sequence (Version 1) |
Description
This biobrick is one of the final constructs in Project Peptidor by Team TU Delft iGEM 2013 to check the sensing of AIP by E.coli and then production of the peptides followed by TetR production to activated the next set of the constructs.Notes
The Biobrick Bba_K1022104 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119Source
Clonned| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1022119 BBa_K206000 promoter AraI1 AraI2 BBa_I746100 B0034-sequence RBS BBa_I746103 agrC coding sequence BamHI site B0034-sequence RBS agrA coding sequence BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_I746104 34 HIs 6 - SUMO Maximin H5 34 TetR B0010 B0012 BBa_K1022120 | 1,2299 1,130 1,131 40,57 61,78 139,2058 139,150 139,2299 157,1404 1405,1410 1411,1422 1429,2058 2067,2146 2078,2121 2155,2195 2162,2181 2182,2195 2188,2188 2204,2299 2300,2313 2314,2634 2635,2698 2703,2706 2720,3335 3410,3489 3498,3538 1,3538 | feature/BioBrick engineered_region engineered_region feature/BioBrick feature/promoter promoter feature/operator operator feature/operator operator engineered_region feature/BioBrick feature/rbs ribosome_entry_site feature/BioBrick engineered_region feature/cds CDS sequence_feature feature/misc feature/rbs ribosome_entry_site CDS feature/cds engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site feature/stop stop_codon feature/BioBrick engineered_region ribosome_entry_site feature/rbs tag feature/tag sequence_feature feature/dna feature/rbs ribosome_entry_site CDS feature/cds stop_codon feature/stop stop_codon feature/stop feature/BioBrick engineered_region |