Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Weifan Liang
Date created: 2013-09-06 11:00:00
Date modified: 2015-05-08 01:08:45
iGEM-mut part
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1025002_sequence (Version 1) |
Description
iGEMmut part is a plasmid constructed by 2013 iGEM Tsinghua-E team which is used for genome level in vivo high-diversity library construction. In this vector, highly error-prone dnaQ mutant, mutD1 was cloned downstream of araBAD promoter so that we can control the mutation intensity of the target genome by the change of the concentration of araBAD promoter???s inducer, L-arabinose. Actually, we measured the mutation increase induced by our mut part with the protocol described in our note from (website citation). To be brief, by measuring the reversion of rifampinresistance caused by mutation in genome, we accurately determined the mutation rate increase to be as big as times compared with negative control. The data is shown below. After the test, we successfully applied our mut part in our iGEM tryptophan overproduction evolution project and obtained a titer increase from to.Notes
We use the backbone of pRedET and add our genes dowmstream the promoter.Source
The mut gene comes from genome of E.coli W3110 with two animo acid mutation. And the promoter comes from pRedET plasmid.| Sequence Annotation | Location | Component / Role(s) |
| araBAD promoter RBS_1 | 1,289 290,304 | feature/promoter promoter ribosome_entry_site feature/rbs |