BBa_K1026001

BBa_K1026001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1026001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hongyi WU

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:08:46

dCas9

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1026001_sequence (Version 1)

Description

dCas9 is a mutant of Cas9, with its endonuclease activity wiped out. dCas9 acts as the sole protein in CRISPRi system (Qi et al., 2013).

Reference
QI, LEI S., LARSON, MATTHEW H., GILBERT, LUKE A., DOUDNA, JENNIFER A., WEISSMAN, JONATHAN S., ARKIN, ADAM P. & LIM, WENDELL A. 2013. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell, 152, 1173-1183.

Notes

Mutation: Aspartate 10 to Alanine (D10A) and Histidine 840 to Alanine (H840A)
in order to cripple the endonuclease activity.

Source

The dCas9 comes from S. pyogenes.

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1026001/1

Member of these Collections

• //function/crispr
• //function/crispr/cas9
• iGEM Parts Registry