BBa_K1027002

BBa_K1027002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1027002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Robert Harrison, Lorna Hepworth, Vun Hiang Tan
Date created: 2013-09-18 11:00:00
Date modified: 2015-05-08 01:08:46

Delta 12 Desaturase, optimised for expression in Escherichia coli



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1027002_sequence (Version 1)

    Description

    This enzyme is obtained from the gene Oleic acid desaturase (ode).The gene is obtained from Synechocystis sp., a cyanobacterium and introduced in the E.coli. It is a trans-membrane enzyme, involved in the biosynthesis of C18:2 from C18:1. C18:2 corresponds to Linolenic acid. The delta 12 desaturase introduces a second cis carbon double bond on the 12th carbon in C18:1.

    Notes

    Codon optimization for expression in E coli

    Source

    Produced synthetically based on protein sequence from Synechocystis sp.

    Sequence Annotation Location Component / Role(s)
    Start
    Stop
    		1,3
    1057,1062
    		feature/start start_codon
    feature/stop stop_codon

    igem#experience
    Works
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1027002/1
     

    Attachments

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