Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Huang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
J23100->tetA-asv+sfGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1030000_sequence (Version 1) |
Description
We use a strong synthetic constitutive promoter, J23100 to drive the expression of tetA, an tetracycline transporter that can also transport toxic nickel ion into cell which reduce e coli growth rate and kill cells. an asv degradation tag is added to the c-terminal of tetA. A superfolder GFP is expressed under the control of the same promoter so that we can use fluorescent plate reader, flow cytometer and microscope to monitor the expression level of tetA. They are not fused.Notes
We tuned the expression level of tetA using a series of synthetic constitutive promoters. Different levels of tetA will make E coli vulnerable at different level of extracellular Nickel ion, as it is transported into cell via tetA.Source
J23100 is an synthetic constitutive promoter from iGEMtetA is an tetracycline transporter from pBR322
sfGFP is superfolder GFP, from iGEM
| Access | Instance | Definition |
| public public public public | BBa_J23100 BBa_B0034 BBa_J31006 BBa_I746916 | BBa_J23100 BBa_B0034 tetA(C)b BBa_I746916 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23100 BBa_B0034 BBa_J31006 BBa_I746916 | 1,35 44,55 64,1254 1261,1980 | BBa_J23100 BBa_B0034 tetA(C)b BBa_I746916 |