BBa_K1030001

BBa_K1030001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1030001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Huang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46

J23101->tetA-asv+sfGFP



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1030001_sequence (Version 1)

Description

We use a synthetic constitutive promoter j23100 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.

Notes

We use a series of synthetic constitutive promoter to tune the expression of tetA, which in turn tune the extracellular Nickel tolerance level. sfGFP is used to monitor tetA expression.

Source

J23101 and sfGFP are from iGEM, tetA is from pBR322.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1030001/1