Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wei Huang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:46
J23101->tetA-asv+sfGFP
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1030001_sequence (Version 1) |
Description
We use a synthetic constitutive promoter j23100 to drive the expression of tetA, and sfGFP. tetA could transport extracellular Nickel ion into cell and nickel is toxic to E coli. An asv degradation tag is added to the c-terminal of tetA. sfGFP is used to monitor tetA expression in real time using fluorescent plate reader, flow cytometer and microscope.Notes
We use a series of synthetic constitutive promoter to tune the expression of tetA, which in turn tune the extracellular Nickel tolerance level. sfGFP is used to monitor tetA expression.Source
J23101 and sfGFP are from iGEM, tetA is from pBR322.| Access | Instance | Definition |
| public public public public | BBa_J23101 BBa_B0034 BBa_J31006 BBa_I746916 | BBa_J23101 BBa_B0034 tetA(C)b BBa_I746916 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23101 BBa_B0034 BBa_J31006 BBa_I746916 | 1,35 44,55 64,1254 1261,1980 | BBa_J23101 BBa_B0034 tetA(C)b BBa_I746916 |