BBa_K1030008

BBa_K1030008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1030008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wei Huang

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:08:46

Zeo-resistant gene + GFPuv

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1030008_sequence (Version 1)

Description

We use a zeocin resistant gene and external zeocin to tune the cell growth rate. The common antibiotic resistant genes are reserved for plasmid backbones. GFPuv is used to monitor the expression of zeocin resistant gene. In addition, zeocin resistant gene allosterically inhibitor the effect of zeocin, makes it easy to be quantified and mathematically modeled.

Notes

We want to use signalling molecule produced by E coli to tune zeo-r expression, and its growth rate withzeo presents.

Source

Zeo is from a commercial plasmid. GFPuv is from iGEM

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
GFP protein	1,720	CDS, feature/protein
BBa_E0040	1,720	feature/BioBrick, engineered_region

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1030008/1

Member of these Collections

• iGEM Parts Registry