Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: He Shuaixin
Date created: 2013-08-31 11:00:00
Date modified: 2015-05-08 01:08:48
XylS-Terminator
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1031911_sequence (Version 1) |
Description
XylS senses benzoate and its substitutes, it is the transcriptional activation factor that activates the expression of meta-operon for benzoate degradation on TOL plasmid pWW0 in Pseudomonas putida.This part contains XylS coding sequence with terminator. To complete the sensor, please add promoter before XylS coding sequence.
Notes
Molecular cloning to add terminator to XylS coding sequence.Source
XylS comes from Pseudomonas putida, sequence found through Genbank: http://www.ncbi.nlm.nih.gov/nuccore/M10143.1| Sequence Annotation | Location | Component / Role(s) |
| ATG start coden XylS CDS B0015 TGA stop coden | 1,3 1,966 974,1103 964,966 | start_codon feature/start feature/cds CDS feature/BioBrick engineered_region stop_codon feature/stop |