BBa_K1031921

BBa_K1031921 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1031921

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Zheng Pu

Date created: 2013-09-22 11:00:00

Date modified: 2015-05-08 01:08:48

Pm promoter

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1031921_sequence (Version 1)

Description

The cognate promoter regulated by XylS, Pm, is σ70-dependent in E.coli; meanwhile in Pseudomonas putida, it is σ54-dependent. It acts as the master regulator to control the ON/OFF expression of meta-operon on TOL plasmid pWW0. In this meta-operon, XylXYZLTEGFJQKIH genes encode enzymes for the degradation of benzoate and its derivatives, generating intermediate products in TCA cycle

Notes

Pm promoter+ eGFP, we didn't use sfGFP because the intensity of eGFP is more suitable for our biosensor.

Source

Pseudomonas putida, TOL pWW0 plasmid

Components

Access	Instance	Definition
public	BBa_E0840	GFP genera

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E0840	1,878	GFP genera

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1031921/1

Member of these Collections

• iGEM Parts Registry