BBa_K1033204

BBa_K1033204 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1033204
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Viktor Blomkvist
Date created: 2013-08-22 11:00:00
Date modified: 2015-05-08 01:08:49

pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K1033204_sequence (Version 1)

Description

E. coli Lactobacilli shuttle vector pSBLb4C15 is a shuttle vector containing the ori pSH71 originating from the plasmid pJP059 which was taken from Lactococcus lactis. It has a red insert.

Notes

Had to make sure it was compatible with biobrick standard 10

Source

Ori in pEL4C15 replaced with ori from pJP059. Chloramphenicol resistance replaced with erythromycin resistance.

Sequence Annotation Location Component / Role(s)
mrfp1
BBa_E1010
Help:Barcodes
BBa_K1033200
1,675
1,706
682,706
1,706
CDS feature/cds
engineered_region feature/BioBrick
feature/barcode engineered_tag
engineered_region feature/BioBrick
igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1033204/1