| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1033260_sequence (Version 1)
|
Description
This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. See http://mic.sgmjournals.org/content/151/2/421.long.
Notes
The part still contains two illegal restriction sites for EcorI and SpeI. However since it doesn't matter where in your construct you put the system it can still be assembled with XbaI and PstI.
Source
The Lactobacillus plantarum NC7 plasmid p256.
