Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jama Hagi-Yusuf
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:08:50
Bxb1 Integrase and Recombination Directionality Factor expression cassette
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1039007_sequence (Version 1) |
Description
A strong promoter and strong RBS (BBa_K608002) drive expression of Bxb1 integrase and Bxb1 recombination directionality factor (RDF) (BBa_K1039003 and BBa_K1039006). The RBS (BBa_B0034) is associated with RDF.Together, Bxb1 integrase and RDF (BBa_K1039003 and BBa_K1039006) catalyze site-specific recombination between Bxb1 attR and attL sites.
They will also allow flipping of Bxb1 invertible promoter switches BBa_K1039004 and BBa_K1039004 from RL state to PB state.
Notes
Bxb1 integrase (BBa_K1039003) has a silent mutation at base 1269, change from A to T, a silent mutation at base 273 (of gene), change G->A, still codes Gln and a silent mutation at base 1440 (of gene), change G->A, still codes Gln.Source
Parts to create this expression cassette were synthesized by BioBasic Inc.| Sequence Annotation | Location | Component / Role(s) |
| Bxb1 RDF Bxb1 integrase scar BBa_B0034 scar scar BBa_K608002 | 1589,2356 62,1564 1583,1588 1571,1582 1565,1570 56,61 1,55 | sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |