BBa_K1039013

BBa_K1039013 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1039013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jama Hagi-Yusuf

Date created: 2013-09-10 11:00:00

Date modified: 2015-05-08 01:08:50

PhiC31 Recombination Directionality Factor

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1039013_sequence (Version 1)

Description

This is the recombination directionality factor (RDF) that acts with PhiC31 serine integrase. It reverses the recombination directionality of PhiC31 integrase (BBa_K1039012)

In concert with PhiC31 RDF, PhiC31 integrase (BBa_K1039012) catalyzes site-specific recombination between PhiC31 attR and attL sites, to produce PhiC31 attP and attB sites. This recombination is unideirectional, i.e. attP and attB cannot be recombined to produce attL and attR in the presence of PhiC31 integrase and RDF.

In concert with PhiC31 RDF (BBa_K1039013), PhiC31 integrase can be used to flip the PhiC31 invertible promoter switches (BBa_K1039010 and BBa_K1039011) from RL state to PB state.

Notes

Added base after the stop codon to prevent creation of an SpeI site due to addition of suffix

Source

The sequence was obtained by Genbank and was synthesized by BioBasic Inc

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• rdf-schema#seeAlso: Q9T216
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1039013/1

Member of these Collections

• iGEM Parts Registry