Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jama Hagi-Yusuf
Date created: 2013-09-10 11:00:00
Date modified: 2015-05-08 01:08:50
Bxb1 Invertible Strong Promoter Switch Test Construct
| Types | DnaRegion |
| Roles | DNA sequence_feature |
| Sequences | BBa_K1039025_sequence (Version 1) |
Description
Bxb1 invertible promoter switch (BBa_K1039001) in PB state with gfp gene (BBa_K1039015) on the prefix side of the switch and rfp gene (BBa_J04450) on the suffix side. GFP is produced in this state of the switch.The invertible promoter is strong promoter J23119.
Notes
To have expression on the prefix side of the switch, an "inverted" message must be used. The promoter of the switch is facing the prefix side and promotes the ORF to be read from suffix to prefix. In this case, "inverted" means the ORF is read from suffix to prefix.Source
The switches and iGFP were synthesized by BioBasic inc.| Access | Instance | Definition |
| public public public | BBa_K1039015 BBa_K1039001 BBa_K093005 | BBa_K1039015 BS1 RBS-RFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1039015 BBa_K1039001 BBa_K093005 | 1,740 749,1291 1300,2023 | BBa_K1039015 BS1 RBS-RFP |