BBa_K1039025

BBa_K1039025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1039025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jama Hagi-Yusuf

Date created: 2013-09-10 11:00:00

Date modified: 2015-05-08 01:08:50

Bxb1 Invertible Strong Promoter Switch Test Construct

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K1039025_sequence (Version 1)

Description

Bxb1 invertible promoter switch (BBa_K1039001) in PB state with gfp gene (BBa_K1039015) on the prefix side of the switch and rfp gene (BBa_J04450) on the suffix side. GFP is produced in this state of the switch.

The invertible promoter is strong promoter J23119.

Notes

To have expression on the prefix side of the switch, an "inverted" message must be used. The promoter of the switch is facing the prefix side and promotes the ORF to be read from suffix to prefix. In this case, "inverted" means the ORF is read from suffix to prefix.

Source

The switches and iGFP were synthesized by BioBasic inc.

Components

Access	Instance	Definition
public	BBa_K1039015	BBa_K1039015
public	BBa_K1039001	BS1
public	BBa_K093005	RBS-RFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1039015	1,740	BBa_K1039015
BBa_K1039001	749,1291	BS1
BBa_K093005	1300,2023	RBS-RFP

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1039025/1

Member of these Collections

• iGEM Parts Registry