BBa_K1039027

BBa_K1039027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1039027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jama Hagi-Yusuf

Date created: 2013-09-12 11:00:00

Date modified: 2015-05-08 01:08:50

PhiC31 Invertible Strong Promoter Switch Test Construct

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K1039027_sequence (Version 1)

Description

Phic31 invertible promoter switch (BBa_K1039008) in PB state with gfp gene (BBa_K1039015) on the prefix side of the switch and rfp gene (BBa_J04450) on the suffix side. GFP is produced in this state of the switch.

The invertible promoter is strong promoter J23119.

Notes

Note that there is an NheI site on the prefix side of the switch before the attP site. This was placed there as part of an early design of our switch, but does not contribute to functionality.

Source

The parts were either synthesized by BioBasic Inc or from the parts registry

Components

Access	Instance	Definition
public	BBa_K1039015	BBa_K1039015
public	BBa_K1039008	BBa_K1039008
public	BBa_K093005	RBS-RFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1039015	1,740	BBa_K1039015
BBa_K1039008	749,1263	BBa_K1039008
BBa_K093005	1272,1995	RBS-RFP

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1039027/1

Member of these Collections

• iGEM Parts Registry