BBa_K1041002

BBa_K1041002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1041002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: NRP UEA

Date created: 2013-08-14 11:00:00

Date modified: 2015-05-08 01:08:51

AntG Promoter + RFP Coding Device

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K1041002_sequence (Version 1)

Description

Created using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.

Notes

-

Source

BBa_K1041000 BBa_K1041001

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
S. S4 AntG Promoter	24,62	feature/promoter, promoter
rbs	113,121	feature/rbs, ribosome_entry_site
NdeI site	124,129	feature/misc, sequence_feature
mrfp1	127,801	feature/cds, CDS
BBa_E1010	127,832	engineered_region, feature/BioBrick
Help:Barcodes	808,832	engineered_tag, feature/barcode
BBa_B0010	841,920	engineered_region, feature/BioBrick
BBa_B0015	841,969	engineered_region, feature/BioBrick
stem_loop	852,895	feature/stem_loop, stem_loop
BBa_B0012	929,969	feature/BioBrick, engineered_region
T7 TE	939,955	feature/stem_loop, stem_loop
polya	956,969	feature/polya, polyA_site
stop	962,962	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1041002/1

Member of these Collections

• iGEM Parts Registry