BBa_K105006

BBa_K105006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Karstens

Date created: 2008-10-11 11:00:00

Date modified: 2015-05-08 01:08:52

lacI - DNA binding domain

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K105006_sequence (Version 1)

Description

The LacI protein is a repressor that binds to a specific operator site ([[Part:BBa_R0010| BBa_R0010]] & [[Part:BBa_K105023| BBa_K105023]]). This site specific binding can be inhibited by Lactose or its analog ITPG.

This BioBrick contains only the DNA binding domain of the natural LacI protein.

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.

For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

We changed the format of the lacI coding part of [[Part:BBa_C0012| BBa_C0012]] into Phillips/Silver standard.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105006/1

Member of these Collections

• //cds/dnabinding
• iGEM Parts Registry