Types | DnaRegion
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Roles | Coding
CDS
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Sequences | BBa_K105008_sequence (Version 1)
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Description
This is an aquivalent to [[Part:BBa_J63001| J63001]]. We reconstructed this BioBrick because the length of the part we received from the registery didn't correspond to the J63001. Neither the sequence from the MIT sequencing did.
Notes
To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.
For more information about this issus, see:
Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).
Source
We changed the format of the EYFP coding part of BBa_E2020 into Phillips/Silver standard.