ECFP

BBa_K105009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K105009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Katja Karstens

Date created: 2008-10-11 11:00:00

Date modified: 2015-05-08 01:08:52

ECFP, yeast optimized for fusion proteins

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K105009_sequence (Version 1)

Description

Our BioBrick is cyan fluorescent protein which can be used to label proteins in yeast. Therefor it has specific characteristics of BioBrick part for fusion proteins (see design details).

Notes

To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.

For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

We changed the format of the ECFP coding part of [[Part:BBa_E2020|BBa_E2020]] into Phillips/Silver standard.

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K105009/1

Member of these Collections

• //chassis/eukaryote/yeast
• //function/reporter
• //function/reporter/fluorescence
• iGEM Parts Registry