BBa_K105016

BBa_K105016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K105016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Wild, Katja Karstens
Date created: 2008-10-21 11:00:00
Date modified: 2015-05-08 01:08:52

Leu2 - Beta-isopropylmalate dehydrogenase of yeast



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K105016_sequence (Version 1)

Description

The Beta-isopropylmalate dehydrogenase (IMDH) catalyzes the third step in the leucine biosynthesis pathway of S. cerevisiae. Under the name of Leu2 it is one of the commonly used auxotrophic markers in Yeast. There excists a number of S. cerevisiae strains which are deleated for this enzyme.

Our BioBrick contains the protein coding sequence as well as the natural promoter and terminator of the gene. To avoid a transcription of genes downstream of this part, it might be usefull put a strong termintor (i.e. Adh1_term [[Part:BBa_J63002| BBa_J63002]]) in between the parts.

Notes

Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
So there is no base in between the BioBrick and the restriction sites.


For more information about this issus, see:

Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).

Source

Amplification from the plasmid pRS315 (Sikorski & Hieter)

Sequence Annotation Location Component / Role(s)
Beta-isopropylmalate dehydrogenase
please mutate A -> G
660,1755
1298,1298
feature/cds CDS
feature/s_mutation point_mutation
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K105016/1