BBa_K105023

BBa_K105023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K105023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Katja Karstens
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:52

lac I - operator



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K105023_sequence (Version 1)

Description

This BioBrick is the lac I recognition site. It can be used for the construction of promoters which should be regulated by activators or repressors containing a lac I DNA binding domain ([[Part:BBa_K105006| BBa_K105006]]).





Notes

This BioBrick contains the consensus sequence plus 5 spacer nucleotides on each side. The spacer is directly followed by the restriction site without a base in between. So when you construct multiple operator sites from this basic part you will have 16 bp between two consensus sequences.

Source

from oligos

Sequence Annotation Location Component / Role(s)
consensus sequence
6,20
feature/conserved conserved_region
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K105023/1