Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K105026_sequence (Version 1)
|
Description
This is an aquivalent to [[Part:BBa_J63006| J63006]]. We reconstructed this BioBrick because the length of the part we received from the registery didn't correspond to the J63006. Neither the sequence from the MIT sequencing did.
Notes
This BioBrick ends with a Kozak sequence. To enable the direct fusion with DNA coding domains in frame the vector of this BioBrick has no base pair in between the restriction sites and the BioBrick.
For more information about this issus, see:
Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering."
DSpace http://hdl.handle.net/1721.1/32535 (2006).
Source
yeast genomic DNA