BBa_K105027

BBa_K105027 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K105027
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Wild
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:08:52

cyc100 minimal promoter



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K105027_sequence (Version 1)

Description

This is the core of the CYC1 promoter of S. cerevisiae. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery.



Notes

This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!

Source

Amplification from the plasmid pAH3.

Sequence Annotation Location Component / Role(s)
TATA box
17,23
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K105027/1