Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K105031_sequence (Version 1)
|
Description
This is a derivate of [[Part:BBa_K105027| BBa_K105027]]. Due to a point mutation in the TATA box region the efficiency of this minimal promoter is only 16 % of the natural yeast CYC1 promoter.
Notes
This BioBrick contains the TATA-box with one point mutation and most of the 5'UTR (-139 to -36, relative to the translational start) of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added!
Even if there isn't any need for this BioBrick to be fusible to other protein domains, we stayed with our design.
Source
Amplification and Mutation from the plasmid pAH3