BBa_K1051301

BBa_K1051301 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1051301

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kequan Lin

Date created: 2013-06-29 11:00:00

Date modified: 2015-05-08 01:08:54

clb2 promoter (during G2 phase)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1051301_sequence (Version 1)

Description

It is a promoter which promotes transciption in G2 of the yeast cell cycle .If you want to express your proteins in G2 of the yeast cell cycle ,you can add the cln2 sequence before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation .

Notes

We get the sequence of the cln2 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows :
upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTAGCATCTCATAGCATGGG
downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTACTATAAGATCAATGAAGAGA

Source

CTAGC ATCTC ATAGC ATGGG CATAGAGTTTCAGAAATTTTGCTCTTAATGGAAAATATAA
CCTCTTTGGGGAAAAGAGAAATAGATCCTTTCTCAAAAATTTAGAAAGGAAAAAAAATCA
AAATATAATAAAATAGATTTTTTTTAGGTTAATTTTTTGCCAATTTAGCTAGACTTTTGA
AAAGATCCAAAAATTTACTAAAATTGAATCTTTCTGGTATTAATTTTGTCCTATACCTCA
AGAAGTAAAGTATTCTCGAATTGATACTTCTGAAAAGCGAAAGAAATATAGCTAAAGGAT
ACTGGTTATTTATTCTTTCTTTTTTTCTTTCTTTTTGATTGAGCCAGTGTATAATAAAAA
CCTACTGCGAGCTATTCAATAAATTGACATAGAATATCTGTTGAAGCGGTTCTTTGATTG
AGCATCTGTTTTCCTTTTGTATTTTTGTCCTTTTAGATTCTTTTAACGACATTGGAGAAA
TTTTAATCATTGTATAAAAACTTCATTCGCTCGTTTGTCAGAAGTTTTTTTATAACTTGC
TATTTCCTTCCAAGAAGCCTTTTATTGATTACCCCCTCTCTCTCTTCATTGATCTTATAG

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1051301/1

Member of these Collections

• iGEM Parts Registry