BBa_K1053015

BBa_K1053015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1053015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ippei Sakamoto

Date created: 2013-09-26 11:00:00

Date modified: 2015-05-08 01:08:56

PBAD-TR(12)-HHR-taR12y-DT

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K1053015_sequence (Version 1)

Description

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12. In absence of taR12, the HHR can self-cleave, but in presence of taR42, their capability of self-cleavage is inhibited due to hybridize with taR12 and change their structures. This HHR has taR12y in the downstream. taR12 and taR12y are orthogonal riboregulators reported by Callura et al.

Notes

BBa_K1053012 & BBa_K1053010
Overlap PCR was performed to connect BBa_K1053012 and taR12-DT in BBa_K1053010.

Source

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR42. In absence of taR42, the HHR can self-cleave, but in presence of taR42, their capability of self-cleavage is inhibited due to hybridize with taR42 and change their structures. This HHR has taR12 in the downstream. taR12 and taR42 are orthogonal riboregulators reported by Callura et al.

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1053015/1

Member of these Collections

• iGEM Parts Registry