BBa_K1053016

BBa_K1053016 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1053016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ippei Sakamoto
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:08:56

PBAD-TR(12y)-HHR-taR42-DT



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1053016_sequence (Version 1)

Description

This parts is the taRNA (taR12) generator which can be repressed by taRNA (taR42).

Notes

BBa_K1053012 & BBa_K1053009
Overlap PCR was performed to connect BBa_K1053012 and taR12-DT in BBa_K1053009.

Source

This hammerhead ribozyme(HHR) has trans-acting RNA(taRNA) responsive region, and this HHR responses to taR12y. In absence of taR12y, the HHR can self-cleave, but in presence of taR12y, their capability of self-cleavage is inhibited due to hybridize with taR12y and change their structures. This HHR has taR42 in the downstream. taR12y and taR42 are orthogonal riboregulators reported by Callura et al.

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1053016/1