BBa_K1059011

BBa_K1059011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1059011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjun Wang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:57

RBS J23106+mamL coding squence



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1059011_sequence (Version 1)

Description

mamL is a protein coding squence from Magnetospirillum magneticum AMB-1 bacteria strain, mamAB gene cluster. It will guide the construction of MMB with mamI, mamB, mamQ, mamK, which we use to construct the intracellular compartment.

Notes

Because synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.

Source

It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS B0032, prefix and sufix onto it.

Sequence Annotation Location Component / Role(s)
RBS-3\Weak
BBa_B0032
RBS
1,13
1,13
7,10
feature/rbs ribosome_entry_site
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1059011/1