Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wenjun Wang
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:08:57
RBS B0032+mamB coding squence
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1059013_sequence (Version 1) |
Description
mamB is a protein coding squence from Magnetospirillum magneticum AMB-1 bacteria strain, mamAB gene cluster. It will guide the construction of MMB with mamI, mamQ, mamL, mamK, which we use to construct the intracellular compartment.Notes
Because there is a PstI cut site, so we process point mutation by over-lap PCR.synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.Source
It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it.| Sequence Annotation | Location | Component / Role(s) |
| RBS-3\Weak BBa_B0032 RBS BBa_K1059091 | 1,13 1,13 7,10 14,904 | ribosome_entry_site feature/rbs engineered_region feature/BioBrick ribosome_entry_site feature/rbs feature/protein CDS |