Types | DnaRegion
|
Roles | CDS
Coding
|
Description
mamB is a protein coding squence from Magnetospirillum magneticum AMB-1 bacteria strain, mamAB gene cluster. It will guide the construction of MMB with mamI, mamQ, mamL, mamK, which we use to construct the intracellular compartment.
Notes
Because synthetic biology proposes standard prefix and sufix, so we add prefix and sufix by PCR before we ligease it onto plasmid PSB1C3.
Source
It comes from Magnetospirillum magneticum AMB-1 bacteria strain.We firstly design primers to get this gene squence by PCR from genome, and then use another primer to add RBS J23106, prefix and sufix onto it.