| Types | DnaRegion
|
| Roles | Coding
CDS
|
| Sequences | BBa_K1060000_sequence (Version 1)
|
Description
The aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also BBa_K1060003). As basic chorismate levels inside E. coli are limited. We wanted to upregulate the biosynthesis of chorismate. The basic AroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.
Notes
no EcoRI, XbaI, NotI, SpeI or PstI sites present
Source
Escherichia coli K-12 (DH5α) genomic DNA
