BBa_K1060011

BBa_K1060011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1060011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ingmar Claes, Lukas Marcelis, Su Wang

Date created: 2013-09-18 11:00:00

Date modified: 2015-05-08 01:08:58

Beta Farnesene production

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K1060011_sequence (Version 1)

Description

For the production of the alarmhormone,(E)-β-farnesene, a sesquiterpene synthase gene (GenBank Accession No. AJ271793) was put behind a medium (constitutive) promoter and medium RBS (BBa_K608006). Additionally a Lac operator sequence (Gilbert & Maxam, 1973) was introduced to regulate its expression.

Notes

no EcoRI, XbaI, NotI, SpeI or PstI sites present

Source

EBF synthase from Artemisia annua genome (Picaud et al., 2005; BBa_K1060002), Lac operator (Gilbert & Maxam, 1973)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
K608006	1,57	feature/BioBrick, engineered_region
lacZo	56,88	feature/operator, operator
K1060002	95,1828	feature/BioBrick, engineered_region
B0015	1837,1965	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1060011/1

Member of these Collections

• iGEM Parts Registry