Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sergio Peisajovich
Date created: 2008-10-23 11:00:00
Date modified: 2015-05-08 01:08:58
AarI AD acceptor vector (pRS315, Cyc1P, Adh1t)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K106014_sequence (Version 1) |
Description
Acceptor vector for AarI based cloning. Accepts any combination of parts beginning with an A site and ending with a D site. Expression of construct is driven by a Cyc1 promoter, with Adh1 terminator.Notes
The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.Please note, the actual orientation of this vector is reversed.
Source
A modified pRS315 plasmid--CEN ARS yeast expression vector (LEU2 marker).| Sequence Annotation | Location | Component / Role(s) |
| CEN ARS ampicillin resistance marker Cyc1 Promoter D overhang Adh1 terminator LEU2 marker A overhang T7 promoter lacZalpha reporter f1 origin ampicillin resistance promoter pBR322 origin lac promoter T3 promoter Stop codons in all 3 frames Start codon | 3403,3921 4053,4911 6132,6394 1,4 23,216 1570,2676 6394,6397 224,242 246,405 427,733 3983,4011 5068,5687 5996,6025 6095,6114 11,21 6385,6387 | sequence_feature feature/misc feature/cds CDS feature/promoter promoter feature/dna sequence_feature feature/stem_loop stem_loop CDS feature/cds feature/dna sequence_feature promoter feature/promoter sequence_feature feature/misc feature/dna sequence_feature promoter feature/promoter feature/dna sequence_feature feature/promoter promoter promoter feature/promoter stop_codon feature/stop feature/start start_codon |