Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Dinh
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:08:58
Guide RNA (gRNA) target for GFP
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1062001_sequence (Version 1) |
Description
This part is a guide RNA (gRNA) that targets GFP, when bound to Cas9. It also contains a cleavage site recognized by the Csy4 enzyme which allows it to be cleaved from neighboring DNA/RNA.Notes
none?Source
The GFP gRNA was retrieved from Stanley Qi's Lab at UCSF.| Sequence Annotation | Location | Component / Role(s) |
| Csy4 Cleavage Site target sequence to GFP Cas9 hairpin gRNA Terminator | 1,20 21,40 41,83 84,123 | feature/misc sequence_feature feature/binding non_covalent_binding_site feature/misc sequence_feature stop_codon feature/stop |