BBa_K1065104

BBa_K1065104 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1065104
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Gabriele Girelli
Date created: 2013-08-12 11:00:00
Date modified: 2015-05-08 01:08:59

RBS + SAM synthetase



    • Details

    Types
    DnaRegion

    Roles
    Translational_Unit

    engineered_region

    Sequences BBa_K1065104_sequence (Version 1)

    Description

    SAM synthetase enzyme catalyzes the reaction of synthesis of S-AdoMet. RBS is 'AAGGAGG'.

    Notes

    The gene sequence was extracted using the following primers:

    >Primer Forward: prefix + RBS + spacer + ATG...
    GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

    >Primer Reverse: sequence_TAATAA_suffix
    GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

    Source

    SAM synthetase gene sequence extracted from E. coli MG1655 strain genome.

    Sequence Annotation Location Component / Role(s)
    RBS
    spacer
    SAM synthetase
    		1,7
    8,14
    15,1169
    		ribosome_entry_site feature/rbs
    feature/misc sequence_feature
    feature/cds CDS

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1065104/1
     

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