Types | DnaRegion
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Roles | Composite
engineered_region
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Sequences | BBa_K1065303_sequence (Version 1)
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Description
YF1, a blue light-sensing protein, works together with FixJ, its RR. Phosphorilated FixJ binds FixK2 promoter regulating its activity.
The part presents RBS-coupled YF1 and FixJ but it doesn't present any terminator.
Notes
FixK2 is regulated by phospho-FixJ, so the promoter had to be put downstream from BBa_K592016.
Source
YF1 is a fusion protein of YtvA (from B subtilis) and FixL (from B japonicum); FixJ and FixK2 come from the genome of B. japonicum.
The part has been constructed using two parts from the registry though: BBa_k592016 and BBa_k592006.