BBa_K1065303

BBa_K1065303 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1065303
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fabio Digiacomo
Date created: 2013-08-12 11:00:00
Date modified: 2015-05-08 01:08:59

B0034-YF1-B0034-FixJ-pFixK2



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1065303_sequence (Version 1)

Description

YF1, a blue light-sensing protein, works together with FixJ, its RR. Phosphorilated FixJ binds FixK2 promoter regulating its activity.
The part presents RBS-coupled YF1 and FixJ but it doesn't present any terminator.

Notes

FixK2 is regulated by phospho-FixJ, so the promoter had to be put downstream from BBa_K592016.

Source

YF1 is a fusion protein of YtvA (from B subtilis) and FixL (from B japonicum); FixJ and FixK2 come from the genome of B. japonicum.
The part has been constructed using two parts from the registry though: BBa_k592016 and BBa_k592006.

igem#experience
None
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1065303/1