Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Horwitz
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:08:59
AarI AD acceptor, pRS315, 8X LexAOPs Cyc1P, Adht1
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K106670_sequence (Version 1) |
Description
This is an acceptor vector for AarI cloning with 8X Lex operators directly upstream of a Cyc1 promoter. The vector contains the AarI cloning cassette (AD sites, with Adh1 terminator). It is a pRS315 derived vector, for CEN ARS expression in cerevisiae (LEU2 marker).Notes
LexA operator repeats were cloned into the PspOMI site at the 5' end of the Cyc1 promoter. The inner site was broken, thus the operator-promoter-AarI cassette fragment can be subcloned by PspOMI/SacI or Kpn1/SacI digestion into any other pRS series vector.Source
This is a derivative of part BBa_K106014, the AarI AD acceptor vector (pRS315, Cyc1P, Adh1t).| Sequence Annotation | Location | Component / Role(s) |
| CEN ARS ampicillin resistance marker 8X LexA Operators Cyc1 Promoter ampicillin resistance promoter Adh1 terminator LEU2 Marker D overhang Stop codons in all 3 frames A overhang T7 promoter lacZalpha reporter f1 origin pBR322 origin lac promoter T3 promoter ATG start codon | 3403,3921 4053,4911 6132,6299 6300,6556 3983,4011 23,216 1570,2676 1,4 11,21 6556,6559 224,242 246,405 427,733 5068,5687 5996,6025 6095,6114 6547,6549 | sequence_feature feature/misc CDS feature/cds operator feature/operator feature/promoter promoter feature/promoter promoter feature/stem_loop stem_loop CDS feature/cds sequence_feature feature/dna stop_codon feature/stop feature/dna sequence_feature feature/promoter promoter feature/misc sequence_feature feature/dna sequence_feature feature/dna sequence_feature promoter feature/promoter promoter feature/promoter start_codon feature/start |