Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andrew Horwitz
Date created: 2008-10-28 12:00:00
Date modified: 2015-05-08 01:09:00
AarI A!D acceptor vector (pRS315, Cyc1P, Adh1t)
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K106693_sequence (Version 1) |
Description
This is a special variant acceptor vector for AarI cloning. The "A" site used here, termed "A!" is an alternate end used when no amino-terminal fusions are tolerated. Please see the UCSF 2008 iGEM wiki for more information on AarI cloning.This was designed specifically for use with Sir3 and amino-terminal domains of Sir3.
Notes
Note that this vector will NOT accept standard AarI "A" parts.Source
Modified pRS315 plasmid.| Sequence Annotation | Location | Component / Role(s) |
| LEU2 marker CEN ARS ampicillin resistance marker Adh1P! promoter AarI A!D MCS Adh1 terminator T7 promoter lacZalpha reporter f1 origin ampicillin resistance promoter pBR322 origin lac promoter T3 promoter Adh1 promoter D overhang A overhang Start codon Stop codons in all 3 frames | 1570,2676 3403,3921 4053,4911 6132,7642 4305,4381 18,211 224,242 246,405 427,733 3983,4011 5068,5687 5996,6025 6095,6114 7229,7625 1,4 7647,7650 7638,7640 11,21 | CDS feature/cds sequence_feature feature/misc feature/cds CDS promoter feature/promoter sequence_feature feature/misc stem_loop feature/stem_loop feature/promoter promoter sequence_feature feature/misc sequence_feature feature/dna feature/promoter promoter sequence_feature feature/dna promoter feature/promoter promoter feature/promoter feature/promoter promoter feature/dna sequence_feature feature/dna sequence_feature feature/start start_codon feature/stop stop_codon |