BBa_K1076002

BBa_K1076002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1076002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Carlos Gustavo Nunes
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:03

FadL, Long-chain fatty acid outer membrane transporte



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1076002_sequence (Version 1)

Description

Gene of fatty acid transport protein, outer membrane; long-chain fatty acid receptor. We are expressing this full lenght gene in order to enhance the long chain fatty acids intake by Shewanella putrefaciens to increase B-oxidation and therefore ATP and H+ production.

Notes

We chosed the full lenght gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3. Further subclonning and expression should be done within pSB3C5

Source

From Shewanella putrefaciens genomic sequence

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1076002/1