Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_K1076003_sequence (Version 1)
|
Description
Gene of fatty acid transport protein, outer membrane; long-chain fatty acid receptor. We are expressing this coding sequence (ORF) in order to enhance the long chain fatty acids intake by Shewanella putrefaciens to increase B-oxidation and therefore ATP and H+ production.
Notes
We chosed this ORF gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3.
Source
From Shewanella putrefaciens genomic sequence