BBa_K1076003

BBa_K1076003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1076003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Carlos Gustavo Nunes
Date created: 2013-09-12 11:00:00
Date modified: 2015-05-08 01:09:03

FadD, Long-chain-fatty-acid-CoA ligase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1076003_sequence (Version 1)

Description

Gene of fatty acid transport protein, outer membrane; long-chain fatty acid receptor. We are expressing this coding sequence (ORF) in order to enhance the long chain fatty acids intake by Shewanella putrefaciens to increase B-oxidation and therefore ATP and H+ production.

Notes

We chosed this ORF gene to be expressed into Shewanella putrefaciens making a super expression since the bacteria already have one copy of this gene in its genome. We made the primers pair with EcoR1 (5') and Pst1 (3') ends in order to clone into pSB1C3.

Source

From Shewanella putrefaciens genomic sequence

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1076003/1