BBa_K1081000

BBa_K1081000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1081000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Hangxing Jia

Date created: 2013-09-09 11:00:00

Date modified: 2015-05-08 01:09:04

J13002-dcas9

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1081000_sequence (Version 1)

Description

Cas9 is an RNA-guided double-stranded DNA endonuclease, which belongs to the type II bacterial CRISPR system, from Streptococcus pyogenes. Dead cas9(dcas9), in which two mutations were introduced to abolish
the endonuclease function, is added behind the biobrick J13002. Here we combine dcas9 with several different guide RNAs to activate or repress one of the double promoters??? transcription.

Notes

We made point mutation of EcoRI Restriction Enzyme cutting site inside the coding region of dcas9.

Source

Dcas9 was provided by addgene. Cas9 is a gene from Streptococcus pyogenes.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J13002	1,74	feature/BioBrick, engineered_region
R0040	1,54	sequence_feature, feature/misc
BBa_K1081000	1,4180	engineered_region, feature/BioBrick
B0034	63,74	feature/misc, sequence_feature
dcas9	77,4180	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1081000/1

Member of these Collections

• //function/crispr
• //function/crispr/cas9
• iGEM Parts Registry