Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: S. P. van Kessel
Date created: 2013-09-10 11:00:00
Date modified: 2015-06-08 04:01:14
pSB1AC3-HySp: Integrational backbone into B. subtilis amyE locus with IPTG inducible promoter
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K1085014_sequence (Version 1) |
Description
We used the amyE intergrational backbone form Munich 2012 (BBa_K823023) that had no promoter. We've added the Hy_Spank IPTG inducable promoter from Rudner's Lab 2004. The Hy_Spank promoter is right in front of the prefix so any biobrick can be inserted in this backbone.Notes
In pDR111 the Hy_spank promoter and the lacI gene are orientated in the same direction. In order to have the Hy_spank promoter right in front of the prefix we turend the promoter around.Source
iGEM Munich 2012 (Rudner Lab 2004) and Rudner's Lab 2004 (pDR111)| Sequence Annotation | Location | Component / Role(s) |
| Hy_Spank amyE amyE lacI amp cat lacO lacO -10 -35 suffix prefix Term. (-14.4 kcals) Term. (-12.8 kcals) | 7497,7588 1379,2407 5546,6059 6096,7178 4361,5221 358,1008 7497,7516 7568,7588 7558,7558 7533,7533 1,21 7604,7625 314,350 7435,7462 | feature/promoter promoter feature/misc sequence_feature sequence_feature feature/misc feature/cds CDS feature/cds CDS feature/cds CDS operator feature/operator feature/operator operator feature/misc sequence_feature sequence_feature feature/misc feature/dna sequence_feature sequence_feature feature/dna stem_loop feature/stem_loop stem_loop feature/stem_loop |