BBa_K1087000

BBa_K1087000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1087000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tong Xu
Date created: 2013-08-27 11:00:00
Date modified: 2015-05-08 01:09:06

RBS-cre-ssrA(LAA)tag



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K1087000_sequence (Version 1)

Description

This is a cre coding region with RBS and a ssrA(LAA) tag,which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

Notes

no

Source

This part was PCRed from BBa_I718008 to include RBS and cre,while the reverse primer bring the ssrA(LAA) tag to the end of cre coding sequence.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1087000/1