Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-07-08 11:00:00
Date modified: 2015-05-08 01:09:06
Reporter system to quantify protein expression of B. sub 1-deoxyxylulose-5-phosphate synthase (Dxs)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1088002_sequence (Version 1) |
Description
We constructed a system to quantify the protein expression of our desired gene, Dxs, by using the amilCP reporter. The system is under transcriptional control by a sigma 70 dependent promoter and we have used the terminator present in the pSB1C3 plasmid. The Dxs gene is translationally fused to the amilCP gene and therefore the stopcodon of dxs have been removed. The Dxs gene was amplified with primers that carried both RBS and the promoter in its overhang. Also a glycine linker was added between dxs and amilCP.Notes
We did not include the stop codon of the Dxs gene in order to fuse them translationally with the amilCP gene.The Dxs gene was amplified with primers that carried both RBS and the promoter in its overhang. Also a glycine linker was added between dxs and amilCP.
Source
Go to basic parts for origin of DNA| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1088000 promoter | 35,1901 1,35 | feature/BioBrick engineered_region feature/promoter promoter |