BBa_K1088004

BBa_K1088004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andreas Kj??r

Date created: 2013-08-05 11:00:00

Date modified: 2015-05-08 01:09:06

IspG (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1088004_sequence (Version 1)

Description

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) is a part of the bacterial MEP pathway used for production of isoprenoids.

From ecocyc:
"IspG catalyzes the conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate. IspG activity depends on as-yet unidentified additional proteins, most likely involved in the oxidation portion of the reaction"
http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10370

Notes

We designed primers to isolate IspG directly from the genome by colony PCR. The primers included an overhang containing the biobrick prefix and suffix

Source

Isolated from E. coli MG1655

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ispG	1,1119	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• rdf-schema#seeAlso: A1AE53
• rdf-schema#seeAlso: A7ZPV8
• rdf-schema#seeAlso: B1IWE6
• rdf-schema#seeAlso: B1XAZ0
• rdf-schema#seeAlso: C4ZX90
• rdf-schema#seeAlso: P62620
• rdf-schema#seeAlso: P62621
• rdf-schema#seeAlso: P62622
• rdf-schema#seeAlso: Q0TEX0
• rdf-schema#seeAlso: Q1R8L8
• rdf-schema#seeAlso: B5Z0Y4
• rdf-schema#seeAlso: B6I587
• rdf-schema#seeAlso: B7LDA7
• rdf-schema#seeAlso: B7LKC3
• rdf-schema#seeAlso: B7MI00
• rdf-schema#seeAlso: B7MYF0
• rdf-schema#seeAlso: B7NRG5
• rdf-schema#seeAlso: B7UGW1
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088004/1

Member of these Collections

• iGEM Parts Registry