Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Patrick Rosendahl Andreassen
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:06
LacI-LVA (part C0012 without barcodes)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1088023_sequence (Version 1) |
Description
See BBa_C0012Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.
A rapid degradation tail (LVA) has been added to improve the switch time for High to Low performance of this part.
Notes
Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.Incompatible with systems containing LacI, lactose, or IPTG.