pLac

BBa_K1088054 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1088054

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Patrick Rosendahl Andreassen

Date created: 2015-03-29 11:00:00

Date modified: 2015-05-08 01:09:06

Lac operator

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1088054_sequence (Version 1)

Description

This part is an inverting regulator sensitive to LacI and CAP.

It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.


In the absence of LacI protein and CAP protein, this part promotes transcription.
In the presence of LacI protein and CAP protein, this part inhibits transcription.
LacI can be inhibited by IPTG.
LacI is coded by BBa_C0010

Notes

No

Source

pKT25

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Lac promoter	88,118	promoter, feature/promoter
CAP binding	52,73	non_covalent_binding_site, feature/binding
Lac operator	126,142	feature/operator, operator

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1088054/1

Member of these Collections

• iGEM Parts Registry