Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jakub Piątkowski
Date created: 2013-09-20 11:00:00
Date modified: 2015-05-08 01:09:08
HBB+CsfGFP m6 (+B0034)
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1093021_sequence (Version 1) |
Description
Human hemoglobin beta chain fused at C-terminus with the C-terminal fragment of superfolder green fluorescent protein. m6 mutated version of the C-terminal sfGFP fragment offers larger sensitivity at the price of lowers specificity (as opposed to m12 vaersion) Along with BBa_K1093020 and BBa_K1093031 it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to inhibit proper assembly of hemoglobin tetramer.Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.
Notes
HBB and sfGFP are fused with a flexible linker BBa_K157009Source
The sequence was synthetized by GeneRay| Sequence Annotation | Location | Component / Role(s) |
| HBB-CsfGFP(m6) B0034 start stop B0010+B0012 | 19,561 1,12 19,21 562,567 577,704 | CDS feature/protein ribosome_entry_site feature/rbs feature/start start_codon feature/stop stop_codon feature/stem_loop stem_loop |