Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yin-Chih Chen, LIANG-CHUN CHEN
Date created: 2013-10-26 11:00:00
Date modified: 2015-05-08 01:09:09
RFP-FimH(mannose-binding domain) fusion protein
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1104101_sequence (Version 1) |
Description
FimH protein, a protein expressed in Escherichia coli K-12 substr MG1655, has a mannose-binding site from the 22nd to 179th amino acid. FimH protein is originally located on the tip of the fimbria of the E. coli, which will adhere to the mannose on the host's cell membrane, allowing the bacteria to colonize various host tissues. In our project we managed to use it to bind the mannose polymer, which serves as a sticky end to bind the host cell, on top of the PTP1, in order to stop the adhesion between the polar filament and the epithelial cell of the bee's midgut.Notes
whether the RFP will properly expressSource
Escherichia coli K-12 substr MG1655 genomic sequence| Sequence Annotation | Location | Component / Role(s) |
| RFP(without stop codon) FimH mannose-binding domain stop codon | 1,675 676,1149 1149,1180 | CDS feature/cds feature/cds CDS feature/stop stop_codon |